thr77 for Dummies
thr77 for Dummies
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are described. Two more splice variants have been reported but specific nucleotide sequence details is not still accessible.
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Blocking peptides are peptides that bind specifically towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This information outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best benefits are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to be certain the absolute best success are...
Antibodies sure to the blocking peptide no more bind on the epitope on the focus on protein. By evaluating the staining within the blocked antibody versus the antibody on your own, you can see which staining is precise.
Target Details Cyclin E belongs on the very conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show unique expression and degradation styles which contribute into the temporal coordination of each and every mitotic party. Cyclin E varieties a fancy with and features being a regulatory subunit website of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates on the G1-S phase boundary and it is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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